USB Port Lock Software For Windows

BuduLock

BuduLock is a simple USB port lock software for Windows which can also be used to lock folders. It does not entirely serves the function to lock USB port, but blocks USB flash drives and memory drives. Other devices such as mouse, keyboard, webcam, etc. will work properly when the lock is active.
There are two tabs available, one lets you lock USB port, while other lets you lock folders. Enabling the USB flash lock will not let your computer access any kind of USB memory drives. The change password option lets you change the password for extra security. To lock folders, navigate to the folders tab and select the folder which you want to be locked.

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Disable USB via RegEdit

Disable USB via REGEDIT to lock USB port. You will have to access your computer's registry to carry out this process. Let me remind you that if you do not handle Registry properly and end up changing wrong values, your computer may not boot. So, you have to be very careful while working with REGEDIT.

How to block USB port through registry:

Go to Start menu and type Run to access the Run box.
Type regedit and click on OK to open the Registry Editor.
Navigate to the following location in the Registry Editor window: HKEY_LOCAL_MACHINE\SYSTEM\CurrentControlSet\Services\USBSTOR.
In the USBSTOR folder, double click on Start.
A new window will pop up where you can change the value of that registry.
The default value is set to 3; change it to 4 and click Ok.
Exit Registry Editor and reboot the computer for the change to take place.

After you have restarted your computer, you will not be able to access USB storage devices through USB. To enable USB again, repeat the same steps and change the value back to 3. It is the easiest way to lock USB port, but you need to be very careful while handling Registry Editor.

Nomesoft USB Guard

Nomesoft USB Guard is another simple USB port blocker for Windows. It has a very simple interface and provides standard USB blocking options. You can either entirely block USB detection, or make the USB device read only. The primary options on the UI lets you Enable the Use of USB or Disable the use of USB.
When you disable USB port, it stops reading USB flash drives instantly; however, it will not block other USB devices such as mouse, keyboard, printer, etc. Enabling USB ports let you choose if you want to make the USB device a read only, or a read-write device. This USB port locker software works perfectly fine and is easy to use.
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USBDeview

USBDeview is a USB port manager which lets you enable and disable USB port lock. With this freeware to lock USB port, you can view the list of USB ports along with the devices connected to them. To enable or disable USB port, select the port from the list of ports which you want to make changes to, then click on Red button to disable and Green button to enable. It is one of the best USB port blocker software as it lets you specifically block USB ports.
Another good feature of this freeware is that it lets you block USB port of another computers remotely.
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Ratool

Ratool is another free USB port lock software which allows you to fully allow, read only, and block USB ports. The interface lets you choose from 3 actions:

Allow Read & Write (Default)
Allow Read-only
Block USB Storage Devices

Other features let you add a password to change settings, allow/deny access to all removable devices, and disable autorun. You can also set permission measures for CD/DVD and Floppy devices. This USB port blocker is simple and free to use.
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Flash Drive Permission

Flash Drive Permission lets you block USB ports of Windows. It has a tiny interface with only 4 options. These options let you set No Restriction, Read only Restriction, and No Access Restriction. It only sets restriction on USB storage devices. There are no other options such as password protection, etc.
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USB Disabler

USB Disabler is a simple open source USB port lock software. It has just 2 options: Disable USB Flash
and Enable USB Flash. The UI displays the status of this USB locker if its enabled or disabled.
PS: Open this freeware in Run as Administrator Mode, else it will not work.
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USB SHUTTER

USB SHUTTER works perfectly as an USB port blocker software. Click on the Enable/Disable toggle button to switch between the enable USB and disable USB mode. The status is displayed on the UI.
Run as Administrator to make this USB blocker work. The status of USB blocker is carried forward even when the computer is restarted. Open the software and change the status in the software from blocked to unblocked to access USB or vice versa.
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Phrozen Safe USB

Phrozen Safe USB lets you block USB port on Windows. This free USB locker lets you set the USB Device fully operational, USB Device in read only mode, and USB Device deactivated.
Select any of the above three options to activate it. You can also choose to start this USB Port lock with Windows. Minimize it to keep it running in background.
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